Improvement of Alignment Accuracy in Electron Tomography

MAFFT version 5: improvement in accuracy of multiple sequence alignment

The accuracy of multiple sequence alignment program MAFFT has been improved. The new version (5.3) of MAFFT offers new iterative refinement options, H-INS-i, F-INS-i and G-INS-i, in which pairwise alignment information are incorporated into objective function. These new options of MAFFT showed higher accuracy than currently available methods including TCoffee version 2 and CLUSTAL W in benchmar...

Improvement in the accuracy of multiple sequence alignment program MAFFT.

In 2002, we developed and released a rapid multiple sequence alignment program MAFFT that was designed to handle a huge (up to approximately 5,000 sequences) and long data (approximately 2,000 aa or approximately 5,000 nt) in a reasonable time on a standard desktop PC. As for the accuracy, however, the previous versions (v.4 and lower) of MAFFT were outperformed by ProbCons and TCoffee v.2, bot...

Automatic Alignment of Electron Tomography Images Using Markers

Markov random field based automatic image alignment for electron tomography.

We present a method for automatic full-precision alignment of the images in a tomographic tilt series. Full-precision automatic alignment of cryo electron microscopy images has remained a difficult challenge to date, due to the limited electron dose and low image contrast. These facts lead to poor signal to noise ratio (SNR) in the images, which causes automatic feature trackers to generate err...

Accuracy Improvement of Tropospheric Delay Interpolation in RTK Networks

The effect of troposphere on the signals emitted from global navigation satellite system (GNSS) satellites, appears as an extra delay in the measurement of the signal traveling from the satellite to receiver. This delay depends on the temperature, pressure, humidity as well as the transmitter and receiver antennas location. In GNSS positioning, tropospheric delay effects on accuracy of differen...